More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| O-6-methylguanine-DNA methyltransferase | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MGMT |
Protein | Methylated-DNA--protein-cysteine methyltransferase |
Uniprot ID | P16455 |
Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | 6 O methylguanine DNA methyltransferase antibody|6-O-methylguanine-DNA methyltransferase antibody|Agat antibody|AGT antibody|AI267024 antibody|Methylated DNA protein cysteine methyltransferase antibody|Methylated-DNA--protein-cysteine methyltransferase antibody|Methylguanine DNA methyltransferase antibody|MGC107020 antibody|MGMT antibody|MGMT_HUMAN antibody|O 6 methylguanine DNA alkyltransferase antibody|O 6 methylguanine DNA methyltransferase antibody|O-6-methylguanine-DNA methyltransferase antibody|O-6-methylguanine-DNA-alkyltransferase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MGMT antibody, ASA-B1270, Western blotting
All lanes: Anti MGMT (ASA-B1270) at 0.5ug/ml
WB: Recombinant Human MGMT Protein 0.5ng
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti MGMT (ASA-B1270) at 0.5ug/ml
WB: Recombinant Human MGMT Protein 0.5ng
Predicted bind size: 21KD
Observed bind size: 21KD
Anti- MGMT antibody, ASA-B1270, Western blotting
All lanes: Anti MGMT (ASA-B1270) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti MGMT (ASA-B1270) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti- MGMT antibody, ASA-B1270, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue