More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| myocyte enhancer factor 2C | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2C(406-419aa SRYPQHTRHEAGRS), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MEF2C |
Protein | Myocyte-specific enhancer factor 2C |
Uniprot ID | Q06413 |
Function | Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle- specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. |
Tissue Specificity | Expressed in brain and skeletal muscle. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the MEF2 family. |
Aliases | C5DELq14.3 antibody|DEL5q14.3 antibody|MADS box transcription enhancer factor 2 polypeptide C(myocyte enhancer factor 2C) antibody|MEF2C antibody|MEF2C_HUMAN antibody|Myocyte enhancer factor 2C antibody|Myocyte specific enhancer factor 2C antibody|Myocyte-specific enhancer factor 2C antibody|OTTHUMP00000222409 antibody|Similar to MADS box transcription enhancer factor 2 polypeptide C antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEF2C antibody, ASA-B1250, IHC(P)
IHC(P): Human Intestine Tissue
IHC(P): Human Intestine Tissue
Anti-MEF2C antibody, ASA-B1250, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: COLO320 Cell Lysate
