MCM7 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
minichromosome maintenance complex component 7	Polyclonal	IgG	Rabbit	Human	IHC-P, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MCM7(699-719aa DEYEELNVWQVNASRTRITFV).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	MCM7
Protein	DNA replication licensing factor MCM7
Uniprot ID	P33993
Function	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Tissue Specificity
Sub-cellular localization	Nucleus .
Sequence Similarities	Belongs to the MCM family.
Aliases	CDABP0042 antibody|CDC 47 antibody|CDC47 antibody|CDC47 homolog antibody|Cdc47, S. cerevisiae, homolog of antibody|DNA replication licensing factor MCM7 antibody|Homolog of S. cerevisiae Cdc47 antibody|MCM 2 antibody|MCM 7 antibody|MCM2 antibody|MCM2, formerly antibody|Mcm7 antibody|MCM7 minichromosome maintenance deficient 7 antibody|MCM7_HUMAN antibody|Minichromosome Maintainence 7 antibody|Minichromosome maintainence, S. cerevisiae, homolog of antibody|Minichromosome maintenance complex component 7 antibody|Minichromosome maintenance deficient 7 antibody|Minichromosome maintenance protein 7 antibody|P1.1 MCM3 antibody|P1.1-MCM3 antibody|P1CDC47 antibody|P85MCM antibody|PNAS 146 antibody|PNAS146 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information