More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| minichromosome maintenance complex component 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MCM7 |
Protein | DNA replication licensing factor MCM7 |
Uniprot ID | P33993 |
Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. |
Tissue Specificity | |
Sub-cellular localization | Nucleus . |
Sequence Similarities | Belongs to the MCM family. |
Aliases | CDABP0042 antibody|CDC 47 antibody|CDC47 antibody|CDC47 homolog antibody|Cdc47, S. cerevisiae, homolog of antibody|DNA replication licensing factor MCM7 antibody|Homolog of S. cerevisiae Cdc47 antibody|MCM 2 antibody|MCM 7 antibody|MCM2 antibody|MCM2, formerly antibody|Mcm7 antibody|MCM7 minichromosome maintenance deficient 7 antibody|MCM7_HUMAN antibody|Minichromosome Maintainence 7 antibody|Minichromosome maintainence, S. cerevisiae, homolog of antibody|Minichromosome maintenance complex component 7 antibody|Minichromosome maintenance deficient 7 antibody|Minichromosome maintenance protein 7 antibody|P1.1 MCM3 antibody|P1.1-MCM3 antibody|P1CDC47 antibody|P85MCM antibody|PNAS 146 antibody|PNAS146 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MCM7 antibody, ASA-B1241, Western blotting
All lanes: Anti MCM7 (ASA-B1241) at 0.5ug/ml
WB: Recombinant Human MCM7 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti MCM7 (ASA-B1241) at 0.5ug/ml
WB: Recombinant Human MCM7 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- MCM7 antibody, ASA-B1241, Western blotting
All lanes: Anti MCM7 (ASA-B1241) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 81KD
All lanes: Anti MCM7 (ASA-B1241) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Anti- MCM7 antibody, ASA-B1241,IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue