More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| minichromosome maintenance complex component 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human MCM6(589-605aa ESEDFIVEQYKHLRQRD), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MCM6 |
Protein | DNA replication licensing factor MCM6 |
Uniprot ID | Q14566 |
Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Note: Binds to chromatin during G1 and detach from it during S phase. |
Sequence Similarities | Belongs to the MCM family. |
Aliases | DNA replication licensing factor MCM6 antibody|MCG40308 antibody|Mcm6 antibody|MCM6 minichromosome maintenance deficient 6(MIS5 homolog, S. pombe) antibody|MCM6 minichromosome maintenance deficient 6 antibody|MCM6_HUMAN antibody|Minichromosome maintenance complex component 6 antibody|Minichromosome maintenance deficient(mis5 S. pombe) 6 antibody|Minichromosome maintenance deficient 6 homolog antibody|Minichromosome maintenance protein 6 antibody|Mis5 antibody|MIS5 homolog antibody|p105MCM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MCM6 antibody, ASA-B1238, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: JURKAT Cell Lysate
Anti-MCM6 antibody, ASA-B1238, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-MCM6 antibody, ASA-B1238, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue