More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| minichromosome maintenance complex component 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3(13-27aa REAQRDYLDFLDDEE), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MCM3 |
Protein | DNA replication licensing factor MCM3 |
Uniprot ID | P25205 |
Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the MCM family. |
Aliases | Cervical cancer proto oncogene 5 antibody|DNA polymerase alpha holoenzyme associated P1 antibody|DNA polymerase alpha holoenzyme associated protein P1 antibody|DNA polymerase alpha holoenzyme-associated protein P1 antibody|DNA replication factor MCM3 antibody|DNA replication licensing factor MCM3 antibody|HCC 5 antibody|HCC5 antibody|hRlf beta subunit antibody|Human cervical cancer proto oncogene 5 antibody|MCM 3 antibody|mcm3 antibody|MCM3 minichromosome maintenance deficient 3 antibody|MCM3_HUMAN antibody|MGC1157 antibody|Minichromosome maintenance complex component 3 antibody|Minichromosome maintenance deficient 3 antibody|Minichromosome maintenance protein 3 antibody|P1 h antibody|P1 MCM3 antibody|P1 Protein antibody|P1-MCM3 antibody|P1.h antibody|p102 antibody|P102 protein antibody|Replication licensing factor beta subunit antibody|RLF beta subunit antibody|RLF subunit beta antibody|RLFB antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MCM3 antibody, ASA-B1235, Western blotting
All lanes: Anti MCM3 (ASA-B1235) at 0.5ug/ml
Lane 1: NIH3T3 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
All lanes: Anti MCM3 (ASA-B1235) at 0.5ug/ml
Lane 1: NIH3T3 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
Anti-MCM3 antibody, ASA-B1235, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-MCM3 antibody, ASA-B1235, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue