More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| myeloid cell leukemia 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MCL1 |
Protein | Induced myeloid leukemia cell differentiation protein Mcl-1 |
Uniprot ID | Q07820 |
Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
Tissue Specificity | |
Sub-cellular localization | Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note: Cytoplasmic, associated with mitochondria. |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | Bcl 2 related protein EAT/mcl1 antibody|Bcl-2-like protein 3 antibody|Bcl-2-related protein EAT/mcl1 antibody|BCL2 related antibody|Bcl2-L-3 antibody|BCL2L3 antibody|EAT antibody|Induced myeloid leukemia cell differentiation protein Mcl 1 antibody|Induced myeloid leukemia cell differentiation protein Mcl-1 antibody|MCL 1 antibody|MCL1 antibody|MCL1-ES antibody|Mcl1/EAT antibody|MCL1_HUMAN antibody|MCL1L antibody|MCL1S antibody|MGC104264 antibody|MGC1839 antibody|Myeloid Cell Leukemia 1 antibody|Myeloid cell leukemia ES antibody|myeloid cell leukemia sequence 1 antibody|Myeloid cell leukemia sequence 1 BCL2 related antibody|Myeloid cell leukemia sequence 1 isoform 1 antibody|OTTHUMP00000032794 antibody|OTTHUMP00000032795 antibody|TM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MCL1 antibody, ASA-B1232--1.jpg
All lanes: Anti MCL1 (ASA-B1232) at 0.5ug/ml
WB: Recombinant Human MCL1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti MCL1 (ASA-B1232) at 0.5ug/ml
WB: Recombinant Human MCL1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-MCL1 antibody, ASA-B1232--2.jpg
All lanes: Anti MCL1 (ASA-B1232) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti MCL1 (ASA-B1232) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-MCL1 antibody, ASA-B1232--3.jpg
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue