More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| kinesin family member 2C | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KIF2C |
Protein | Kinesin-like protein KIF2C |
Uniprot ID | Q99661 |
Function | In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. |
Tissue Specificity | Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. |
Sub-cellular localization | Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere. Chromosome, centromere, kinetochore. Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGOL2. |
Sequence Similarities | Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily. |
Aliases | 4930402F02Rik antibody|ESTM5 antibody|KIF 2C antibody|kif2c antibody|KIF2C_HUMAN antibody|Kinesein Family Member 2C antibody|Kinesin family member 2C antibody|kinesin like 6 (mitotic centromere associated kinesin) antibody|Kinesin like 6 antibody|Kinesin like protein 6 antibody|Kinesin like protein KIF2C antibody|Kinesin-like protein 6 antibody|Kinesin-like protein KIF2C antibody|KNS L6 antibody|KNSL 6 antibody|KNSL6 antibody|MCAK antibody|MGC11883 antibody|Mitotic centromere associated kinesin antibody|Mitotic centromere-associated kinesin antibody|OTTHUMP00000010066 antibody|X83316 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MCAK antibody, ASA-B1228, Western blotting
All lanes: Anti MCAK (ASA-B1228) at 0.5ug/ml
WB: Recombinant Human MCAK Protein 0.5ng
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti MCAK (ASA-B1228) at 0.5ug/ml
WB: Recombinant Human MCAK Protein 0.5ng
Predicted bind size: 48KD
Observed bind size: 48KD
Anti- MCAK antibody, ASA-B1228, Western blotting
All lanes: Anti MCAK (ASA-B1228) at 0.5ug/ml
Lane 1: Mouse Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: MM453 Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
All lanes: Anti MCAK (ASA-B1228) at 0.5ug/ml
Lane 1: Mouse Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: MM453 Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
Anti- MCAK antibody, ASA-B1228, IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue