More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| matrilin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Matrilin 2(937-956aa QRLEEMTQRMEALENRLRYR), identical to the related rat sequence and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MATN2 |
Protein | Matrilin-2 |
Uniprot ID | O00339 |
Function | Involved in matrix assembly. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 10 EGF-like domains. |
Aliases | Crtm2 antibody|MATN 2 antibody|MATN2 antibody|MATN2_HUMAN antibody|Matrilin-2 antibody|Matrilin2 antibody|MGC102477 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Matrilin 2 antibody, ASA-B1222, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human MATN2, 35.8KD(162aa tag+Q803-R956)
Lane 1: Recombinant Human MATN2 Protein 10ng
Lane 2: Recombinant Human MATN2 Protein 5ng
Lane 3: Recombinant Human MATN2 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant human MATN2, 35.8KD(162aa tag+Q803-R956)
Lane 1: Recombinant Human MATN2 Protein 10ng
Lane 2: Recombinant Human MATN2 Protein 5ng
Lane 3: Recombinant Human MATN2 Protein 2.5ng
Anti-Matrilin 2 antibody, ASA-B1222, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue
Anti-Matrilin 2 antibody, ASA-B1222, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue