More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serpin peptidase inhibitor, clade B (ovalbumin), member 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SERPINB5 |
Protein | Serpin B5 |
Uniprot ID | P36952 |
Function | Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
Tissue Specificity | Normal mammary epithelial cells. |
Sub-cellular localization | Secreted, extracellular space. |
Sequence Similarities | Belongs to the serpin family. Ov-serpin subfamily. |
Aliases | mammary serine proteinase inhibitor antibody|Maspin antibody|Ovalbumin antibody|Peptidase inhibitor 5 antibody|PI-5 antibody|PI5 antibody|Protease inhibitor 5 antibody|Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody|Serpin B5 antibody|serpin peptidase inhibitor antibody|Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody|SERPINB5 antibody|SPB5_HUMAN antibody|Spi5 antibody|Spi7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MASPIN antibody, ASA-B1221, Western blotting
All lanes: Anti MASPIN (ASA-B1221) at 0.5ug/ml
Lane 1: Mouse Intestine Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti MASPIN (ASA-B1221) at 0.5ug/ml
Lane 1: Mouse Intestine Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Anti- MASPIN antibody, ASA-B1221, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- MASPIN antibody, ASA-B1221, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue