MAP2 Antibody (monoclonal)

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
microtubule-associated protein 2	Monoclonal	IgG	Mouse	Human, Mouse, Rat	IHC-P, WB	Ascites
Immunogen
Rat brain microtubule-associated proteins(MAPs).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.
Concentration	1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	MAP2
Protein	Microtubule-associated protein 2
Uniprot ID	Human(P11137), Mouse(P20357), Rat(P15146)
Function	The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Tissue Specificity
Sub-cellular localization	Cytoplasm, cytoskeleton .
Sequence Similarities	Contains 3 Tau/MAP repeats.
Aliases	MAP2A|MAP2B|MAP2C|MAP, DENDRITE-SPECIFIC

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.5-2μg/ml	Human, mouse, rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	1-2μg/ml	Human, mouse, rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information