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Mannose 6 Phosphate Receptor(Cation independent) Antibody View larger

Mannose 6 Phosphate Receptor(Cation independent) Antibody

ASA-B1212

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
insulin-like growth factor 2 receptor Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Mannose 6 Phosphate Receptor(Cation independent)(2475-2491aa TKLVSFHDDSDEDLLHI), different from the related rat and mouse sequences by one amino acid.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 IGF2R

Protein

 Cation-independent mannose-6-phosphate receptor

Uniprot ID

 P11717

Function

 Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.

Tissue Specificity

Sub-cellular localization

 Lysosome membrane.

Sequence Similarities

 Belongs to the MRL1/IGF2R family.

Aliases

 300 kDa mannose 6 phosphate receptor antibody|300 kDa mannose 6-phosphate receptor antibody|Cation independent mannose 6 phosphate receptor antibody|Cation-independent mannose-6-phosphate receptor antibody|CD222 antibody|CD222 antigen antibody|CI Man 6 P receptor antibody|CI Man-6-P receptor antibody|CI MPR antibody|CI-MPR antibody|CIMPR antibody|IGF 2 receptor antibody|IGF 2R antibody|IGF II receptor antibody|IGF-II receptor antibody|IGF2 receptor antibody|IGF2R antibody|Insulin like growth factor 2 receptor antibody|Insulin like growth factor II receptor antibody|Insulin-like growth factor 2 receptor antibody|Insulin-like growth factor II receptor antibody|M6P R antibody|M6P/IGF2 receptor antibody|M6P/IGF2R antibody|M6PR antibody|MPR 300 antibody|MPR300 antibody|MPRI antibody|MPRI_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Mannose 6 Phosphate Receptor(Cation independent) antibody, ASA-B1212, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Mannose 6 Phosphate Receptor(Cation independent) antibody, ASA-B1212, ICC
ICC: HCT116 Cell
Anti-Mannose 6 Phosphate Receptor(Cation independent) antibody, Western blotting
All lanes: Anti Mannose 6 Phosphate Receptor (Cation independent) (ASA-B1212) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 274KD
Observed bind size: 274KD

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