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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| myelin associated glycoprotein | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MAG(114-132aa KYYFRGDLGGYNQYTFSEH), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAG |
Protein | Myelin-associated glycoprotein |
Uniprot ID | P20916 |
Function | Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. |
Aliases | GMA antibody|MAG antibody|MAG_HUMAN antibody|Myelin associated glycoprotein antibody|Myelin-associated glycoprotein antibody|S MAG antibody|Siglec 4a antibody|Siglec-4a antibody|SIGLEC4A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MAG antibody, ASA-B1210, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Anti-MAG antibody, ASA-B1210, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-MAG antibody, ASA-B1210, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue