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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SMAD family member 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MADH7(375-389aa YSLQRPNDHEFMQQP), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SMAD7 |
Protein | Mothers against decapentaplegic homolog 7 |
Uniprot ID | O15105 |
Function | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). |
Tissue Specificity | Ubiquitous with higher expression in the lung and vascular endothelium. |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta. |
Sequence Similarities | Belongs to the dwarfin/SMAD family. |
Aliases | FLJ16482 antibody|hSMAD 7 antibody|hSMAD7 antibody|MAD(mothers against decapentaplegic Drosophila) homolog 7 antibody|MAD antibody|Mad homolog 7 antibody|MAD homolog 8 antibody|MAD mothers against decapentaplegic homolog 7 antibody|MADH 7 antibody|MADH 8 antibody|MADH6 antibody|MADH8 antibody|Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody|Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody|Mothers against decapentaplegic homolog 7 antibody|Mothers against decapentaplegic homolog 8 antibody|Mothers against DPP homolog 7 antibody|Mothers against DPP homolog 8 antibody|SMA- AND MAD-RELATED PROTEIN 7 antibody|SMAD 7 antibody|SMAD antibody|SMAD family member 7 antibody|SMAD, mothers against DPP homolog 7(Drosophila) antibody|SMAD, mothers against DPP homolog 7 antibody|SMAD6 antibody|SMAD7 antibody|SMAD7_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MADH7 antibody, ASA-B1207, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-MADH7 antibody, ASA-B1207, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: U87 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: U87 Cell Lysate
