MADH7 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
SMAD family member 7	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MADH7(375-389aa YSLQRPNDHEFMQQP), identical to the related rat and mouse sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SMAD7
Protein	Mothers against decapentaplegic homolog 7
Uniprot ID	O15105
Function	Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
Tissue Specificity	Ubiquitous with higher expression in the lung and vascular endothelium.
Sub-cellular localization	Nucleus. Cytoplasm. Note: Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta.
Sequence Similarities	Belongs to the dwarfin/SMAD family.
Aliases	FLJ16482 antibody|hSMAD 7 antibody|hSMAD7 antibody|MAD(mothers against decapentaplegic Drosophila) homolog 7 antibody|MAD antibody|Mad homolog 7 antibody|MAD homolog 8 antibody|MAD mothers against decapentaplegic homolog 7 antibody|MADH 7 antibody|MADH 8 antibody|MADH6 antibody|MADH8 antibody|Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody|Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody|Mothers against decapentaplegic homolog 7 antibody|Mothers against decapentaplegic homolog 8 antibody|Mothers against DPP homolog 7 antibody|Mothers against DPP homolog 8 antibody|SMA- AND MAD-RELATED PROTEIN 7 antibody|SMAD 7 antibody|SMAD antibody|SMAD family member 7 antibody|SMAD, mothers against DPP homolog 7(Drosophila) antibody|SMAD, mothers against DPP homolog 7 antibody|SMAD6 antibody|SMAD7 antibody|SMAD7_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Rat Human, Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information