> >>LYRIC Antibody
LYRIC Antibody View larger

LYRIC Antibody

ASA-B1201

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
metadherin Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human LYRIC recombinant protein (Position: D101-Q270). Human LYRIC shares 94% amino acid (aa) sequence identity with both mouse and rat LYRIC.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 MTDH

Protein

 Protein LYRIC

Uniprot ID

 Q86UE4

Function

 Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF- kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Tissue Specificity

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Sub-cellular localization

 Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane ; Single-pass membrane protein . Cell junction, tight junction . Nucleus, nucleolus . Cytoplasm, perinuclear region. Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

Sequence Similarities

Aliases

 3D3 antibody|3D3/LYRIC antibody|AEG 1 antibody|AEG-1 antibody|AEG1 antibody|Astrocyte elevated gene 1 antibody|Astrocyte elevated gene-1 protein antibody|LYRIC antibody|LYRIC/3D3 antibody|LYRIC_HUMAN antibody|Lysine rich CEACAM1 associated protein antibody|Lysine rich CEACAM1 co isolated protein antibody|Lysine-rich CEACAM1 co-isolated protein antibody|Metadherin antibody|Metastasis adhesion protein antibody|MTDH antibody|Protein LYRIC antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- LYRIC antibody, ASA-B1201, Western blotting
All lanes: Anti LYRIC (ASA-B1201) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Cardiac Muscle Tissue Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: U87 Whole Cell Lysate at 40ug
Lane 7: 22RV1 Whole Cell Lysate at 40ug
Lane 8: PC-12 Whole Cell Lysate
Anti- LYRIC antibody, ASA-B1201,IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Anti- LYRIC antibody, ASA-B1201,IHC(P)
IHC(P): Human Mammary Cancer Tissue

© 2024 Novateinbio.com