More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serine/threonine kinase 11 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human LKB1(421-433aa SSKIRRLSACKQQ), different from the mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | STK11 |
Protein | Serine/threonine-protein kinase STK11 |
Uniprot ID | Q15831 |
Function | Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non- AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316). |
Tissue Specificity | Ubiquitously expressed. Strongest expression in testis and fetal liver. |
Sub-cellular localization | Nucleus. Cytoplasm. Membrane . Mitochondrion. Note: A small fraction localizes at membranes (By similarity). Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to the mitochondrion during apoptosis. Translocates to the cytoplasm in response to metformin or peroxynitrite treatment. PTEN promotes cytoplasmic localization. |
Sequence Similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily. |
Aliases | hLKB1 antibody|Liver kinase B1 antibody|LKB1 antibody|PJS antibody|Polarization related protein LKB1 antibody|Renal carcinoma antigen NY-REN-19 antibody|Serine/Threonine Kinase 11 antibody|Serine/threonine protein kinase 11 antibody|Serine/threonine protein kinase LKB1 antibody|Serine/threonine protein kinase STK11 antibody|Serine/threonine-protein kinase 11 antibody|Serine/threonine-protein kinase LKB1 antibody|Serine/threonine-protein kinase XEEK1 antibody|Stk11 antibody|STK11_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LKB1 antibody, ASA-B1185, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: Rat Embryo Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: Rat Embryo Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Anti-LKB1 antibody, ASA-B1185, IHC(P)
IHC(P): Human Liver Cancer Tissue
IHC(P): Human Liver Cancer Tissue
