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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lipocalin 2 | Polyclonal | IgG | Rabbit | Mouse, Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LCN2 |
Protein | Neutrophil gelatinase-associated lipocalin |
Uniprot ID | P11672 |
Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Secreted . Note: Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. |
Sequence Similarities | |
Aliases | 24p3 antibody|25 kDa alpha 2 microglobulin related subunit of MMP9 antibody|25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody| Alpha 2 microglobulin related protein antibody|HGNC:6526 antibody|HNL antibody|Lcn 2 antibody|Lcn2 antibody|Lipocalin-2 antibody|Lipocalin2 antibody|Migration stimulating factor inhibitor antibody|MSFI antibody|Neutrophil gelatinase associated lipocalin antibody|Neutrophil gelatinase associated lipocalin precursor antibody|Neutrophil gelatinase-associated lipocalin antibody|NGAL antibody|NGAL_HUMAN antibody|Oncogene 24p3 antibody|Oncogenic lipocalin 24p3 antibody|P25 antibody|Siderocalin antibody|siderocalin LCN2 antibody|SV40 induced 24P3 protein antibody| Uterocalin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Rat | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Rat Rat | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Lipocalin 2 antibody, ASA-B1174, Western blotting
All lanes: Anti Lipocalin 2 (ASA-B1174) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti Lipocalin 2 (ASA-B1174) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
Anti- Lipocalin 2 antibody, ASA-B1174, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
Anti- Lipocalin 2 antibody, ASA-B1174, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue