Leptin Receptor Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
leptin receptor	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Leptin Receptor(798-817aa HDHFIPIEKYQFSLYPIFME), different from the related mouse and rat sequences by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	LEPR
Protein	Leptin receptor
Uniprot ID	P48357
Function	Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity).
Tissue Specificity	Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
Sub-cellular localization	Cell membrane; Single-pass type I membrane protein.
Sequence Similarities	Belongs to the type I cytokine receptor family. Type 2 subfamily.
Aliases	CD 295 antibody|CD295 antibody|CD295 antigen antibody|Db antibody|Fa antibody|HuB219 antibody|LEP R antibody|LEP-R antibody|LEPR antibody|LEPR_HUMAN antibody|LEPRD antibody|LEPROT antibody|Leptin receptor antibody|Leptin receptor fatty antibody|Leptin receptor gene related protein antibody|Leptin receptor precursor antibody|OB R gene related protein antibody|OB receptor antibody|OB-R antibody|OB-RGRP antibody|obl antibody|Obr antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Human, Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information