More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S) | Monoclonal | IgG | Mouse | Feline, Human, Pig | IHC-P, WB | Ascites |
Immunogen |
| Human laminin. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LAMA1; LAMA2; LAMB1; LAMB2 |
Protein | Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2 |
Uniprot ID | Human(P25391), Mouse(P19137), Rat(D4A409), Human(P24043), Mouse(Q60675), Rat(F1M614), Human(P07942), Mouse(P02469), Rat(D3ZQN7), Human(P55268), Mouse(Q61292), Rat(P15800) |
Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix, basement membrane. Note: Major component. |
Sequence Similarities | Contains 17 laminin EGF-like domains. |
Aliases | LAMA|Laminin a|LAMM|CLM|LIS5|LAMB1|LAMS|NPHS5|Laminin M chain|Laminin-12 subunit alpha|Laminin-2 subunit alpha|Laminin-4 subunit alpha|Merosin heavy chain|S-LAM beta |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.25-0.5μg/ml | Human, feline, pig | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, feline, pig | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
