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Laminin Antibody

ASA-B1154

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
laminin, gamma 1 (formerly LAMB2)&laminin, gamma 2&laminin, gamma 3 Polyclonal IgG Rabbit Human IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

Peptide mixture of laminin gamma1,2,3(DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 LAMC1&LAMC2&LAMC3

Protein

 Laminin subunit gamma-1&Laminin subunit gamma-2&Laminin subunit gamma-3

Uniprot ID

 P11047&Q13753&Q9Y6N6

Function

 Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Tissue Specificity

Found in the basement membranes (major component).

Sub-cellular localization

 Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

 Contains 11 laminin EGF-like domains.

Aliases

 LAMB 2 antibody|LAMB2 antibody|LAMC 1 antibody|LAMC1 antibody|Laminin B2 chain antibody|Laminin gamma 1 antibody|Laminin gamma 1 chain antibody|MGC87297 antibody|3918 antibody|B2T antibody|BM600 antibody|Cell-scattering factor 140 kDa subunit antibody|CSF 140 kDa subunit antibody|CSF antibody|EBR2 antibody|EBR2A antibody|Epiligrin subunit gamma antibody|Kalinin subunit gamma antibody|Kalinin/nicein/epiligrin 100 kDa subunit antibody|Ladsin 140 kDa subunit antibody|LAMB2T antibody|LAMC2 antibody|LAMC2_HUMAN antibody|Laminin 5 gamma 2 subunit antibody|Laminin B2t chain antibody|Laminin gamma 2 antibody|laminin gamma 2 chain antibody|Laminin subunit gamma-2 antibody|Laminin-5 subunit gamma antibody|LAMNB2 antibody|Large adhesive scatter factor 140 kDa subunit antibody|MGC138491 antibody|MGC141938 antibody|Nicein subunit gamma antibody|NICEIN-100KDA antibody|LAMA 1 antibody|LAMA antibody|LAMA1 antibody|LAMB1 antibody|Lamc3 antibody|LAMC3_HUMAN antibody|Laminin A chain antibody|Laminin alpha 1 antibody|Laminin alpha 1 chain antibody|Laminin B1 chain antibody|Laminin subunit beta 1 antibody|Laminin subunit gamma-3 antibody|Laminin-12 subunit gamma antibody|Laminin-14 subunit gamma antibody|Laminin-15 subunit gamma antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Laminin antibody, ASA-B1154--1.jpg
All lanes: Anti Laminin (ASA-B1154) at 0.5ug/ml
WB: Recombinant Human Laminin Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti-Laminin antibody, ASA-B1154--2.jpg
All lanes: Anti Laminin (ASA-B1154) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 177KD
Observed bind size: 177KD
Anti-Laminin antibody, ASA-B1154--3.jpg
IHC(P): Human Lung Cancer Tissue

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