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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lamin B1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LMNB1 |
Protein | Lamin-B1 |
Uniprot ID | P20700 |
Function | Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. |
Tissue Specificity | |
Sub-cellular localization | Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. |
Sequence Similarities | Belongs to the intermediate filament family. |
Aliases | ADLD antibody|lamin B1 antibody|Lamin-B1 antibody|LMN antibody|LMN2 antibody|LMNB antibody| Lmnb1 antibody|LMNB1_HUMAN antibody| MGC111419 antibody|OTTHUMP00000159218 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Lamin B1 antibody, ASA-B1150, Western blotting
All lanes: Anti Lamin B1 (ASA-B1150) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
All lanes: Anti Lamin B1 (ASA-B1150) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
Anti- Lamin B1 antibody, ASA-B1150, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- Lamin B1 antibody, ASA-B1150, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue