Lamin A/C Antibody View larger

Lamin A/C Antibody

ASA-B1148

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

lamin A/C Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C(455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

LMNA

Protein

Prelamin-A/C

Uniprot ID

P02545

Function

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.

Tissue Specificity

In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.

Sub-cellular localization

Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Note: Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin- A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.

Sequence Similarities

Belongs to the intermediate filament family.

Aliases

70 kDa Lamin antibody|Cardiomyopathy dilated 1A(autosomal dominant) antibody|CDCD1 antibody|CDDC antibody|Charcot Marie Tooth Disease Axonal Type 2B1 antibody|CMD1A antibody|CMT2B1 antibody|EMD2 antibody|FPL antibody|FPLD antibody|FPLD2 antibody|HGPS antibody|IDC antibody|Lamin A/C antibody|Lamin A/C Isoform 1 Precursor antibody|Lamin A/C Isoform 2 antibody|Lamin A/C Isoform 3 antibody|Lamin A/C like 1 antibody|Lamin-A/C antibody|LDP1 antibody|LFP antibody|LGMD1B antibody|Limb girdle muscular dystrophy 1B(autosomal dominant) antibody|LMN 1 antibody|LMN A antibody|LMN C antibody|LMN1 antibody|LMNA antibody|LMNA_HUMAN antibody|LMNC antibody|NY REN 32 antigen antibody|Prelamin A/C antibody|PRO1 antibody|Progeria 1(Hutchinson Gilford Type) antibody|Renal carcinoma antigen NY REN 32 antibody|Renal carcinoma antigen NY-REN-32 antibody|Renal carcinoma antigen NYREN32 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat Mouse, RatAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
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