More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| KIN, antigenic determinant of recA protein homolog (mouse) | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KIN |
Protein | DNA/RNA-binding protein KIN17 |
Uniprot ID | O60870 |
Function | Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro. |
Tissue Specificity | Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level). |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the KIN17 family. |
Aliases | antigenic determinant of recA protein homolog antibody|Binding to curved DNA antibody|BTCD antibody|DNA/RNA-binding protein KIN17 antibody|HsKin17 protein antibody|KIN antibody|KIN antigenic determinant of recA protein homolog mouse antibody|KIN17 antibody|Kin17 protein antibody|KIN17_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- KIN antibody, ASA-B1123, Western blotting
All lanes: Anti KIN (ASA-B1123) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: U20S Whole Cell Lysate at 40ug
Lane 6: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti KIN (ASA-B1123) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: U20S Whole Cell Lysate at 40ug
Lane 6: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- KIN antibody, ASA-B1123, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
IHC(P): Human Oesophagus Squama Cancer Tissue
