More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| E1A binding protein p300 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EP300 |
Protein | Histone acetyltransferase p300 |
Uniprot ID | Q09472 |
Function | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:10733570, PubMed:11430825, PubMed:11701890, PubMed:12402037, PubMed:12586840, PubMed:12929931, PubMed:14645221, PubMed:15186775, PubMed:15890677, PubMed:16617102, PubMed:16762839, PubMed:18722353, PubMed:18995842, PubMed:23415232, PubMed:23911289, PubMed:23934153, PubMed:8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus. |
Sequence Similarities | Contains 1 bromo domain. |
Aliases | CREBBP/EP300 inhibitory protein 1 antibody|Cyclic AMP responsive enhancer binding protein antibody|E1A associated protein p300 antibody|E1A binding protein p300 antibody|E1A-associated protein p300 antibody|EC 2.3.1.48 antibody|EP300 antibody|EP300: E1A binding protein p300 antibody|EP300_HUMAN antibody|Histone acetyltransferase p300 antibody|KAT3B antibody|p300 HAT antibody|RB and P300 binding protein EID 1 antibody|Retinoblastoma protein associated protein antibody|RSTS2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- KAT3B/p300 antibody, ASA-B1103, Western blotting
All lanes: All lanes: Anti KAT3B/p300 (ASA-B1103) at 0.5ug/ml
WB: Recombinant Human KAT3B/p300 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: All lanes: Anti KAT3B/p300 (ASA-B1103) at 0.5ug/ml
WB: Recombinant Human KAT3B/p300 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti- KAT3B/p300 antibody, ASA-B1103, Western blotting
All lanes: Anti KAT3B/p300 (ASA-B1103) at 0.5ug/ml
Lane 1: Colo320 Whole Cell Lysate at 40ug
Lane 2: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 264KD
Observed bind size: 264KD
All lanes: Anti KAT3B/p300 (ASA-B1103) at 0.5ug/ml
Lane 1: Colo320 Whole Cell Lysate at 40ug
Lane 2: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 264KD
Observed bind size: 264KD
Anti- KAT3B/p300 antibody, ASA-B1103, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue