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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| COP9 signalosome subunit 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human JAB1 recombinant protein (Position: M8-S334). Human JAB1 shares 99.4% amino acid (aa) sequence identity with mouse JAB1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | COPS5 |
Protein | COP9 signalosome complex subunit 5 |
Uniprot ID | Q92905 |
Function | Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Note: Nuclear localization is diminished in the presence of IFIT3. |
Sequence Similarities | Belongs to the peptidase M67A family. CSN5 subfamily. |
Aliases | 38 kDa Mov34 homolog antibody|COP9 (constitutive photomorphogenic) homolog subunit 5 antibody|COP9 constitutive photomorphogenic homolog subunit 5 antibody|COP9 signalosome complex subunit 5 antibody|COP9 signalosome subunit 5 antibody|Cop9 subunit 5 antibody|COPS 5 antibody|COPS5 antibody| CSN 5 antibody|CSN5 antibody|JAB 1 antibody|Jun activation domain binding protein 1 antibody|Jun activation domain binding protein antibody|Jun activation domain-binding protein 1 antibody|Jun activation domain-binding protein antibody|MGC3149 antibody|MOV 34 antibody|MOV34 antibody|MOV34 family, 38-KD member antibody|SGN 5 antibody|SGN5 antibody|Signalosome subunit 5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- JAB1 antibody, ASA-B1082, Western blotting
All lanes: Anti JAB1 (ASA-B1082) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti JAB1 (ASA-B1082) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- JAB1 antibody, ASA-B1082, IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue
Anti- JAB1 antibody, ASA-B1082, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue