More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| inositol 1,4,5-trisphosphate receptor, type 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human ITPR3 (70-95aa QYWKAKQTKQDKEKIADVVLLQKLQH), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITPR3 |
Protein | Inositol 1,4,5-trisphosphate receptor type 3 |
Uniprot ID | Q14573 |
Function | Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. |
Tissue Specificity | Expressed in intestinal crypt and villus epithelial cells. |
Sub-cellular localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the InsP3 receptor family. |
Aliases | 4 antibody|5-trisphosphate receptor antibody|5-trisphosphate receptor type 3 antibody| FLJ36205 antibody|Inositol 1 antibody|Inositol 1,4,5 trisphosphate receptor type 3 antibody|InsP3R3 antibody|IP3 receptor antibody|IP3 receptor isoform 3 antibody|IP3R 3 antibody|IP3R antibody|IP3R3 antibody|ITPR 3 antibody|ITPR3 antibody|ITPR3_HUMAN antibody| Type 3 inositol 1 antibody|Type 3 inositol 1,4,5 trisphosphate receptor antibody|Type 3 InsP3 receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ITPR3 antibody, ASA-B1080, Western blotting
All lanes: Anti ITPR3 (ASA-B1080) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 304KD
Observed bind size: 304KD
All lanes: Anti ITPR3 (ASA-B1080) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 304KD
Observed bind size: 304KD
Anti- ITPR3 antibody, ASA-B1080, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- ITPR3 antibody, ASA-B1080, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue