More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| integrin, beta 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human ITGB1 recombinant protein (Position: N527-D728). Human ITGB1 shares 91% and 88% amino acid (aa) sequences identity with mouse and rat ITGB1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITGB1 |
Protein | Integrin beta-1 |
Uniprot ID | P05556 |
Function | Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. |
Tissue Specificity | Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non- proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the integrin beta chain family. |
Aliases | beta 1 subunit antibody|CD 29 antibody|CD29 antibody|CD29 antigen antibody|Fibrinogen Receptor beta subunit antibody|Fibronectin receptor subunit beta antibody|FNRB antibody|Glycoprotein IIa antibody|GP IIa antibody|GPIIA antibody|integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12) antibody|Integrin beta-1 antibody|Integrin VLA 4 subunit beta antibody|integrin VLA-4 beta subunit antibody|Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody|ITB1_HUMAN antibody|ITGB 1 antibody|ITGB1 antibody|MDF2 antibody|MSK12 antibody|OTTHUMP00000019420 antibody|Very Late Activation Protein beta Polypeptide antibody|Very late activation protein, beta polypeptide antibody|VLA BETA antibody|VLA-4 subunit beta antibody|VLA-BETA antibody|VLAB antibody|VLAbeta antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ITGB1 antibody, ASA-B1079--3.jpg
All lanes: Anti-ITGB1(ASA-B1079) at 0.5ug/ml
WB: Hela Whole Cell Lysate at 40ug
Predicted bind size: 88KD
Observed bind size: 130KD
All lanes: Anti-ITGB1(ASA-B1079) at 0.5ug/ml
WB: Hela Whole Cell Lysate at 40ug
Predicted bind size: 88KD
Observed bind size: 130KD
Anti-ITGB1 antibody, ASA-B1079--1.jpg
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-ITGB1 antibody, ASA-B1079--2.jpg
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue