More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| insulin receptor substrate 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequences identity with mouse and rat IRS1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IRS1 |
Protein | Insulin receptor substrate 1 |
Uniprot ID | P35568 |
Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Contains 1 IRS-type PTB domain. |
Aliases | HIRS 1 antibody|HIRS1 antibody|Insulin Receptor Substrate 1 antibody|IRS 1 antibody|IRS-1 antibody|IRS1 antibody|IRS1_HUMAN antibody|OTTHUMP00000164234 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IRS1 antibody, ASA-B1074, Western blotting
All lanes: Anti IRS1 (ASA-B1074) at 0.5ug/ml
WB: Recombinant Human IRS1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti IRS1 (ASA-B1074) at 0.5ug/ml
WB: Recombinant Human IRS1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- IRS1 antibody, ASA-B1074, Western blotting
All lanes: Anti IRS1 (ASA-B1074) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MM453 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 132KD
Observed bind size: 132KD
All lanes: Anti IRS1 (ASA-B1074) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MM453 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 132KD
Observed bind size: 132KD
Anti- IRS1 antibody, ASA-B1074, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue