More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin-1 receptor-associated kinase 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse IRAKM(349-364aa KHLWYMPEEYIRQGRL), identical to the related rat sequence, and different from the related human sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IRAK3 |
Protein | Interleukin-1 receptor-associated kinase 3 |
Uniprot ID | Q8K4B2 |
Function | Inhibits dissociation of IRAK1 and IRAK4 from the Toll- like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. |
Tissue Specificity | Highly expressed in liver and thymus and at lower levels in heart, brain, spleen and kidney. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. |
Aliases | ASRT5 antibody|IL 1 receptor associated kinase M antibody|IL-1 receptor-associated kinase M antibody|IL1 receptor associated kinase M antibody|Interleukin 1 receptor associated kinase 3 antibody|Interleukin 1 receptor associated kinase M antibody|Interleukin-1 receptor-associated kinase 3 antibody|IRAK 3 antibody|IRAK M antibody|IRAK-3 antibody|IRAK-M antibody|IRAK3 antibody|IRAK3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.1-0.5ug/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IRAKM antibody, ASA-B1065, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Anti-IRAKM antibody, ASA-B1065, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-IRAKM antibody, ASA-B1065, IHC(P)
IHC(P): Mouse Liver Tissue
IHC(P): Mouse Liver Tissue