More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| inositol 1,4,5-trisphosphate receptor, type 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IP3 receptor recombinant protein (Position: A2411-A2758). Human IP3 receptor shares 98% and 97% amino acid (aa) sequences identity with mouse and rat IP3 receptor, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITPR1 |
Protein | Inositol 1,4,5-trisphosphate receptor type 1 |
Uniprot ID | Q14643 |
Function | Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5- trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity). |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the InsP3 receptor family. |
Aliases | 4 antibody|5-trisphosphate receptor antibody|5-trisphosphate receptor type 1 antibody|DKFZp313E1334 antibody|DKFZp313N1434 antibody|inositol 1 4 5 triphosphate receptor type 1 antibody|Inositol 1 4 5 trisphosphate Receptor Type 1 antibody|Inositol 1 antibody|Inositol 145-trisphosphate Receptor Type 1 antibody|InsP3R1 antibody|IP3 antibody|IP3 receptor antibody|IP3 receptor isoform 1 antibody|IP3R 1 antibody|IP3R antibody|IP3R1 antibody|ITPR 1 antibody|Itpr1 antibody|ITPR1_HUMAN antibody|SCA15 antibody|SCA16 antibody|SCA29 antibody|Type 1 inositol 1 4 5 trisphosphate receptor antibody|Type 1 inositol 1 antibody|Type 1 InsP3 receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IP3 receptor antibody, ASA-B1060, Western blotting
All lanes: Anti IP3 receptor (ASA-B1060) at 0.5ug/ml
WB: Recombinant Human IP3 receptor Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti IP3 receptor (ASA-B1060) at 0.5ug/ml
WB: Recombinant Human IP3 receptor Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- IP3 receptor antibody, ASA-B1060, Western blotting
All lanes: Anti IP3 receptor (ASA-B1060) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 314KD
Observed bind size: 314KD
All lanes: Anti IP3 receptor (ASA-B1060) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 314KD
Observed bind size: 314KD
Anti- IP3 receptor antibody, ASA-B1060,IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue