Integrin beta 4  Antibody View larger

Integrin beta 4 Antibody

ASA-B1051

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

integrin, beta 4 Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ITGB4

Protein

Integrin beta-4

Uniprot ID

P16144

Function

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.

Tissue Specificity

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

Sub-cellular localization

Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note: Colocalizes with DST at the leading edge of migrating keratinocytes.

Sequence Similarities

Belongs to the integrin beta chain family.

Aliases

CD 104 antibody|CD104 antibody|CD104 antigen antibody|GP150 antibody|Integrin beta 4 subunit antibody|Integrin beta-4 antibody|ITB4_HUMAN antibody|ITG B4 antibody|ITGB 4 antibody|ITGB4 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Integrin beta 4 antibody, ASA-B1051--1.jpg
IHC(P): Human Intestinal Cancer Tissue
Anti-Integrin beta 4 antibody, ASA-B1051--2.jpg
IHC(P): Human Placenta Tissue
Anti-Integrin beta 4 antibody, ASA-B1051--3.jpg
All lanes: Anti-ITGB4(ASA-B1051) at 0.5ug/ml
WB: Recombinant Human Integrin beta4 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD

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