More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| integrin, alpha E(antigen CD103, human mucosal lymphocyte antigen 1, alpha polypeptide) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha E(1153-1168aa KRKYQQLNLESIRKAQ), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITGAE |
Protein | Integrin alpha-E |
Uniprot ID | P38570 |
Function | Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. |
Tissue Specificity | Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the integrin alpha chain family. |
Aliases | A530055J10 antibody|alpha-E1 antibody|alpha-M290 antibody|aM290 antibody|Antigen CD103 antibody|CD 103 antibody|CD103 antibody|CD103 antigen antibody|HML 1 antigen antibody|HML-1 antigen antibody|HML1 antigen antibody|Human mucosal lymphocyte antigen 1 antibody|Human mucosal lymphocyte antigen 1, alpha subunit antibody|Huminae antibody|Integrin alpha E heavy chain antibody|Integrin alpha E light chain antibody|Integrin alpha E, epithelial-associated antibody|Integrin alpha E1 antibody|Integrin alpha IEL antibody|Integrin alpha M290 antibody|Integrin alpha-E heavy chain antibody|Integrin alpha-IEL antibody|Integrin, alpha E(antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody|Integrin, alpha E antibody|ITAE_HUMAN antibody|Itgae antibody|MGC141996 antibody|MGC183601 antibody|Mucosal lymphocyte 1 antigen antibody|Mucosal lymphocyte antigen 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Integrin alpha E antibody, ASA-B1046, IHC(P)
IHC(P): Human Rectal Cancer Tissue
IHC(P): Human Rectal Cancer Tissue
Anti-Integrin alpha E antibody, ASA-B1046,Western blotting
All lanes: Anti Integrin alpha E (ASA-B1046) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
All lanes: Anti Integrin alpha E (ASA-B1046) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
Anti-Integrin alpha E antibody, ASA-B1046, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue