More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| insulin-like 3(Leydig cell) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human INSL3(115-131aa CCLSGCTQQDLLTLCPY), different from the related rat and mouse sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | INSL3 |
Protein | Insulin-like 3 |
Uniprot ID | P51460 |
Function | Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. |
Tissue Specificity | Expressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the insulin family. |
Aliases | INSL 3 antibody|INSL 3 antibody|INSL3 antibody|INSL3_HUMAN antibody|Insulin like 3 antibody|Insulin-like 3 A chain antibody|Ley IL antibody|Ley-I-L antibody|Leydig insulin like peptide antibody|Leydig insulin-like peptide antibody|Relaxin like factor antibody|Relaxin-like factor antibody|RLF antibody|RLNL antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-INSL3 antibody, ASA-B1037, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
