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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 8 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human IL8(77-95aa CLDPKENWVQRVVEKFLKR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL8 |
Protein | Interleukin-8(IL-8) |
Uniprot ID | P10145 |
Function | IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine alpha (chemokine CxC) family. |
Aliases | (Ala-IL-8)77 antibody|(Ser-IL-8)72 antibody|3 10C antibody|310C antibody|AMCF 1 antibody|AMCF1 antibody|b ENAP antibody|Bbeta thromboglobulin like protein antibody|Beta thromboglobulin like protein antibody|C-X-C motif chemokine 8 antibody|CXC chemokine ligand 8 antibody|CXCL 8 antibody|CXCL8 antibody|Emoctakin antibody|GCP 1 antibody|GCP-1 antibody|GCP/IL-8 protein I antibody|GCP/IL-8 protein II antibody|GCP/IL-8 protein III antibody|GCP/IL-8 protein IV antibody|GCP/IL-8 protein V antibody|GCP/IL-8 protein VI antibody|GCP1 antibody|Granulocyte chemotactic protein 1 antibody|IL 8 antibody|IL-8 antibody|IL-8(1-77) antibody|IL-8(9-77) antibody|IL8 antibody|IL8/NAP1 form I antibody|IL8/NAP1 form II antibody|IL8/NAP1 form III antibody|IL8/NAP1 form IV antibody|IL8/NAP1 form V antibody|IL8/NAP1 form VI antibody|IL8_HUMAN antibody|Inteleukin 8 antibody|Interleukin8 antibody|K 60 antibody|K60 antibody|LECT antibody|LUCT antibody|Lymphocyte derived neutrophil activating factor antibody|Lymphocyte-derived neutrophil-activating factor antibody|LYNAP antibody|MDNCF antibody|MDNCF-b antibody|MDNCF-c antibody|MONAP antibody|Monocyte derived neutrophil activating peptide antibody|Monocyte derived neutrophil activating protein antibody|Monocyte derived neutrophil chemotactic factor antibody|Monocyte-derived neutrophil chemotactic factor antibody|Monocyte-derived neutrophil-activating peptide antibody|NAF antibody|NAP 1 antibody|NAP-1 antibody|NAP1 antibody|Neutrophil activating factor antibody|Neutrophil activating peptide 1 antibody|Neutrophil activating protein 1 antibody|Neutrophil-activating factor antibody|Neutrophil-activating protein 1 antibody|Protein 3 10C antibody|Protein 3-10C antibody|SCYB 8 antibody|SCYB8 antibody|Small inducible cytokine subfamily B member 8 antibody|T cell chemotactic factor antibody|T-cell chemotactic factor antibody|TSG 1 antibody|TSG1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL-8 antibody, ASA-B0993, Western blotting
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Anti-IL-8 antibody, ASA-B0993, IHC(P)
IHC(P): Human Appendicitis Tissue
IHC(P): Human Appendicitis Tissue
