More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 7 | Polyclonal | IgG | Rabbit | Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse IL7(26-42aa ECHIKDKEGKAYESVLM). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL7 |
Protein | Interleukin-7(IL-7) |
Uniprot ID | P10168 |
Function | Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | IL 7 antibody|IL-7 antibody|Il7 antibody|Interleukin 7 antibody|Interleukin-7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL7 antibody, ASA-B1030, Western blotting
Lane 1: Recombinant Mouse IL-7 Protein 10ng
Lane 2: Recombinant Mouse IL-7 Protein 5ng
Lane 3: Recombinant Mouse IL-7 Protein 2.5ng
Lane 1: Recombinant Mouse IL-7 Protein 10ng
Lane 2: Recombinant Mouse IL-7 Protein 5ng
Lane 3: Recombinant Mouse IL-7 Protein 2.5ng
Anti-IL7 antibody, ASA-B1030, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
