More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 4 receptor | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human IL4R(219-232aa TKWHNSYREPFEQH). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL4R |
Protein | Interleukin-4 receptor subunit alpha(IL-4 receptor subunit alpha/IL-4R subunit alpha/IL-4R-alpha/IL-4RA) |
Uniprot ID | P24394 |
Function | Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. |
Tissue Specificity | Isoform 1 and isoform 2 are highly expressed in activated T-cells. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the type I cytokine receptor family. Type 4 subfamily. |
Aliases | 582J2.1 antibody|CD124 antibody|IL 4R alpha antibody|IL-4 receptor subunit alpha antibody|IL-4-binding protein antibody|IL-4R subunit alpha antibody|IL-4R-alpha antibody|IL-4RA antibody|IL4-BP antibody|Il4r antibody|IL4R nirs variant 1 antibody|IL4RA antibody|IL4RA_HUMAN antibody|interleukin 4 receptor antibody|Interleukin 4 receptor alpha chain antibody|MGC118473 antibody|sIL4Ralpha/prot antibody|Soluble IL-4 receptor subunit alpha antibody|Soluble IL-4R-alpha antibody|Soluble interleukin-4 receptor subunit alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL4R antibody, ASA-B1025, Western blotting
All lanes: Anti IL4R (ASA-B1025) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 89KD
Observed bind size: 89KD
All lanes: Anti IL4R (ASA-B1025) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 89KD
Observed bind size: 89KD
Anti-IL4R antibody, ASA-B1025, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
