More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 4 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human IL4(72-88aa, AATVLRQFYSHHEKDTR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL4 |
Protein | Interleukin-4(IL-4) |
Uniprot ID | P05112 |
Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the IL-4/IL-13 family. |
Aliases | B cell growth factor 1 antibody|B cell IgG differentiation factor antibody|B Cell Stimulatory Factor 1 antibody|B-cell stimulatory factor 1 antibody|BCGF 1 antibody|BCGF1 antibody|Binetrakin antibody|BSF 1 antibody|BSF-1 antibody|BSF1 antibody|HCGF antibody|Hodgki's Cell Growth Factor antibody|IA Inducing Factor antibody|IGG1 induction factor antibody|IL 4 antibody|IL-4 antibody|IL4 antibody|IL4_HUMAN antibody|Il4e12 antibody|Interleukin 4 antibody|Interleukin 4, isoform 1 antibody|Interleukin-4 antibody|Interleukin4 antibody|Lymphocyte stimulatory factor 1 antibody|Macrophage fusion factor antibody|Mast cell growth factor 2 antibody|MCGF2 antibody|MFF antibody|MGC79402 antibody|Pitrakinra antibody|T cell growth factor 2 antibody|TCGF2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL4 antibody, ASA-B1024, Western blotting
All lanes: Anti IL4 (ASA-B1024) at 0.5ug/ml
Lane 1: Recombinant Human IL4 Protein 10ng
Lane 2: Recombinant Human IL4 Protein 5ng
Lane 3: Recombinant Human IL4 Protein 2.5ng
Predicted bind size: 14KD
Observed bind size: 14KD
All lanes: Anti IL4 (ASA-B1024) at 0.5ug/ml
Lane 1: Recombinant Human IL4 Protein 10ng
Lane 2: Recombinant Human IL4 Protein 5ng
Lane 3: Recombinant Human IL4 Protein 2.5ng
Predicted bind size: 14KD
Observed bind size: 14KD
Anti-IL4 antibody, ASA-B1024, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
