More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 2 receptor, alpha | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human IL2RA(39-57aa MAYKEGTMLNCECKRGFRR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL2RA |
Protein | Interleukin-2 receptor subunit alpha(IL-2 receptor subunit alpha/IL-2-RA/IL-2R subunit alpha/IL2-RA) |
Uniprot ID | P01589 |
Function | Receptor for interleukin-2. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 2 Sushi (CCP/SCR) domains. |
Aliases | CD25 antibody|IDDM10 antibody|IL 2 RA antibody|IL 2 receptor subunit alpha antibodyIL 2R alpha chain antibody|IL 2R subunit alpha antibody|IL-2 receptor subunit alpha antibody|IL-2-RA antibody|IL-2R subunit alpha antibody|IL2 RA antibody|IL2 Receptor alpha antibody|IL2 receptor subunit alpha antibody|IL2-RA antibody|IL2R alpha chain antibody|IL2R antibody|IL2R subunit alpha antibody|IL2RA antibody|IL2RA_HUMAN antibody|Interleukin 2 receptor alpha chain antibody|interleukin 2 receptor subunit alpha antibody|Interleukin 2 receptor, alpha antibody|Interleukin-2 receptor subunit alpha antibody|Ly 43 antibody|Ly43 antibody|OTTHUMP00000019030 antibody|OTTHUMP00000019031 antibody|OTTHUMP00000019032 antibody|p55 antibody|p55 chain antibody|T Cell Growth Factor Receptor antibody|TAC antigen antibody|TCGFR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IL2RA antibody, ASA-B1017, Western blotting
All lanes: Anti IL2RA (ASA-B1017) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 45KD
All lanes: Anti IL2RA (ASA-B1017) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 45KD
Anti- IL2RA antibody, ASA-B1017, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
