More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 16 | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IL16 recombinant protein (Position: S1212-S1332). Human IL16 shares 86% amino acid (aa) sequence identity with mouse IL16. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL16 |
Protein | Interleukin-16 |
Uniprot ID | Q14005 |
Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. |
Tissue Specificity | Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation. |
Sub-cellular localization | Interleukin-16: Secreted. |
Sequence Similarities | Contains 4 PDZ (DHR) domains. |
Aliases | HsT19289 antibody|IL-16 antibody|IL16 antibody|IL16_HUMAN antibody|Interleukin 16 precursor antibody|Interleukin-16 antibody|LCF antibody|Lymphocyte chemoattractant factor antibody|Neuronal interleukin 16 antibody|NIL16 antibody|PrIL16 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IL16 antibody, ASA-B1007, Western blotting
All lanes: Anti IL16 (ASA-B1007) at 0.5ug/ml
WB: Recombinant Human IL16 Protein 0.5ng
Predicted bind size: 17KD
Observed bind size: 17KD
All lanes: Anti IL16 (ASA-B1007) at 0.5ug/ml
WB: Recombinant Human IL16 Protein 0.5ng
Predicted bind size: 17KD
Observed bind size: 17KD
Anti- IL16 antibody, ASA-B1007, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti- IL16 antibody, ASA-B1007, ICC
ICC: Human Cord Blood
ICC: Human Cord Blood