More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 1, alpha | Polyclonal | IgG | Rabbit | Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL1 alpha(256-270aa HLARGLPSMTDFQIS). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL1A |
Protein | Interleukin-1 alpha(IL-1 alpha) |
Uniprot ID | P01582 |
Function | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
Tissue Specificity | |
Sub-cellular localization | Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
Sequence Similarities | |
Aliases | BAF antibody|FAF antibody|Hematopoietin 1 antibody|Hematopoietin-1 antibody|IL 1 alpha antibody|IL 1 antibody|IL 1A antibody|IL-1 alpha antibody|Il-1a antibody|IL1 ALPHA antibody|IL1 antibody|IL1A antibody|IL1A_HUMAN antibody|IL1F1 antibody|ilia antibody|Interleukin 1 alpha antibody|Interleukin 1 alpha precursor antibody|Interleukin-1 alpha antibody|Interleukin1 alpha antibody|LAF antibody|LEM antibody|Preinterleukin 1 alpha antibody|Pro interleukin 1 alpha antibody|Pro interleukin 1 alpha antibody|Prointerleukin 1 alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL1 alpha antibody, ASA-B0994, Western blotting
All lanes: Anti IL1 alpha (ASA-B0994) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Thymus Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
All lanes: Anti IL1 alpha (ASA-B0994) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Thymus Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
Anti-IL1 alpha antibody, ASA-B0994, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
