More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IKBKB |
Protein | Inhibitor of nuclear factor kappa-B kinase subunit beta |
Uniprot ID | O14920 |
Function | Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. |
Tissue Specificity | Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. |
Sub-cellular localization | Cytoplasm. Nucleus. Membrane raft. Note: Colocalized with DPP4 in membrane rafts. |
Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. |
Aliases | I kappa B kinase 2 antibody|I kappa B kinase beta antibody|I-kappa-B kinase 2 antibody|I-kappa-B-kinase beta antibody|IkBKB antibody|IKK 2 antibody|IKK B antibody|IKK beta antibody|IKK-B antibody|IKK-beta antibody|IKK2 antibody|IKKB antibody|IKKB_HUMAN antibody|IMD15 antibody|Inhibitor of kappa light chain gene enhancer in B cells antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody|Inhibitor of nuclear factor kappa B kinase beta subunit antibody|Inhibitor of nuclear factor kappa B kinase subunit beta antibody|Inhibitor of nuclear factor kappa-B kinase subunit beta antibody|MGC131801 antibody|NFKBIKB antibody|Nuclear factor NF kappa B inhibitor kinase beta antibody|Nuclear factor NF-kappa-B inhibitor kinase beta antibody|Nuclear factor of kappa light chain gene enhancer in B cells inhibitor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IKK beta antibody, ASA-B0986, Western blotting
All lanes: Anti IKK beta (ASA-B0986) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: M231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
All lanes: Anti IKK beta (ASA-B0986) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: M231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
Anti- IKK beta antibody, ASA-B0986,IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti- IKK beta antibody, ASA-B0986, Western blotting
All lanes: Anti IKK beta (ASA-B0986) at 0.5ug/ml
WB: Recombinant Human IKK beta Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
All lanes: Anti IKK beta (ASA-B0986) at 0.5ug/ml
WB: Recombinant Human IKK beta Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD