More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| conserved helix-loop-helix ubiquitous kinase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IKK alpha recombinant protein (Position: V411-E745). Human IKK alpha shares 98% amino acid (aa) sequence identity with mouse IKK alpha. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CHUK |
Protein | Inhibitor of nuclear factor kappa-B kinase subunit alpha |
Uniprot ID | O15111 |
Function | Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa- B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. |
Aliases | chuk antibody|CHUK1 antibody|Conserved Helix Loop Helix Ubiquitous Kinase antibody|Conserved helix loop ubiquitous kinase antibody|Conserved helix-loop-helix ubiquitous kinase antibody|I Kappa B Kinase 1 antibody|I Kappa B Kinase Alpha antibody|I-kappa-B kinase 1 antibody|I-kappa-B kinase alpha antibody|IkappaB kinase antibody|IkB kinase alpha subunit antibody|IKBKA antibody|IKK 1 antibody|IKK A antibody|IKK a kinase antibody|IKK-A antibody|IKK-alpha antibody|IKK1 antibody|IKKA antibody|IKKA_HUMAN antibody|Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody|Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody|NFKBIKA antibody|Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody|Nuclear factor NF kappa B inhibitor kinase alpha antibody|Nuclear factor NF-kappa-B inhibitor kinase alpha antibody|Nuclear factor NFkappaB inhibitor kinase alpha antibody|Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody|TCF-16 antibody|TCF16 antibody|Transcription factor 16 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IKK alpha antibody, ASA-B0983--1.jpg
All lanes: Anti IKKA (ASA-B0983) at 0.5ug/ml
WB: Recombinant Human IKKA Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti IKKA (ASA-B0983) at 0.5ug/ml
WB: Recombinant Human IKKA Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-IKK alpha antibody, ASA-B0983--2.jpg
All lanes: Anti IKKA (ASA-B0983) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 85KD
Observed bind size: 85KD
All lanes: Anti IKKA (ASA-B0983) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 85KD
Observed bind size: 85KD
Anti-IKK alpha antibody, ASA-B0983--3.jpg
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue