More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112). Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NFKBIA |
Protein | NF-kappa-B inhibitor alpha |
Uniprot ID | P25963 |
Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
Sequence Similarities | Belongs to the NF-kappa-B inhibitor family. |
Aliases | I kappa B alpha antibody|I(Kappa)B(alpha) antibody|I-kappa-B-alpha antibody|IkappaBalpha antibody|IkB-alpha antibody|IKBA antibody|IKBA_HUMAN antibody|IKBalpha antibody|MAD 3 antibody|MAD3 antibody|Major histocompatibility complex enhancer binding protein MAD3 antibody|Major histocompatibility complex enhancer-binding protein MAD3 antibody|NF kappa B inhibitor alpha antibody|NF-kappa-B inhibitor alpha antibody|NFKBI antibody|NFKBIA antibody|Nuclear factor of kappa light chain gene enhancer in B cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IKB alpha antibody, ASA-B0980, Western blotting
All lanes: Anti IKB alpha (ASA-B0980) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Mouse Kidney Tissue Lysate at 50ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti IKB alpha (ASA-B0980) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Mouse Kidney Tissue Lysate at 50ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- IKB alpha antibody, ASA-B0980, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- IKB alpha antibody, ASA-B0980, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue