More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| insulin-like growth factor binding protein 3 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human IGFBP3(182-198aa IKKGHAKDSQRYKVDYE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IGFBP3 |
Protein | Insulin-like growth factor-binding protein 3(IBP-3/IGF-binding protein 3/IGFBP-3) |
Uniprot ID | P17936 |
Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. |
Tissue Specificity | Expressed by most tissues. Present in plasma. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 IGFBP N-terminal domain. |
Aliases | Acid stable subunit of the 140 K IGF complex antibody|Binding protein 29 antibody|Binding protein 53 antibody|BP 53 antibody|BP53 antibody|Growth hormone dependent binding protein antibody|IBP 3 antibody|IBP-3 antibody|IBP3 antibody|IBP3_HUMAN antibody|IGF binding protein 3 antibody|IGF-binding protein 3 antibody|IGFBP 3 antibody|IGFBP-3 antibody|IGFBP3 antibody|Insulin Like Growth Factor Binding Protein 3 antibody|Insulin-like growth factor-binding protein 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IGFBP3 antibody, ASA-B0976, Western blotting
All lanes: Anti IGFBP3 (ASA-B0976) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: MCF- 7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 40KD
All lanes: Anti IGFBP3 (ASA-B0976) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: MCF- 7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 40KD
Anti-IGFBP3 antibody, ASA-B0976, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
