IGF1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
insulin-like growth factor 1(somatomedin C)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IGF1(80-96 aa GSSSRRAPQTGIVDECC), different from the mouse and rat sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	IGF1
Protein	Insulin-like growth factor I(IGF-I)
Uniprot ID	P05019
Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation.
Tissue Specificity
Sub-cellular localization	Secreted.
Sequence Similarities	Belongs to the insulin family.
Aliases	IBP1 antibody|IGF 1 antibody|IGF I antibody|IGF IA antibody|IGF IB antibody|IGF-I antibody|Igf1 antibody|IGF1_HUMAN antibody|IGFI antibody|IGFIA antibody|Insulin like growth factor 1(somatomedin C) antibody|Insulin like growth factor 1 antibody|Insulin like growth factor IA antibody|Insulin like growth factor IB antibody|Insulin-like growth factor I antibody|Mechano growth factor antibody|MGF antibody|OTTHUMP00000195080 antibody|OTTHUMP00000195081 antibody|OTTHUMP00000195082 antibody|OTTHUMP00000195083 antibody|OTTHUMP00000195084 antibody|Somatomedia C antibody|Somatomedin C antibody|Somatomedin-C antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information