More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| iduronate 2-sulfatase | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Iduronate 2 sulfatase(430-448aa ELCREGKNLLKHFRFRDLE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IDS |
Protein | Iduronate 2-sulfatase |
Uniprot ID | P22304 |
Function | Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. |
Tissue Specificity | Liver, kidney, lung, and placenta. |
Sub-cellular localization | Lysosome. |
Sequence Similarities | |
Aliases | Alpha L iduronate sulfate sulfatase antibody|Alpha-L-iduronate sulfate sulfatase antibody|AW214631 antibody|Ids antibody|IDS_HUMAN antibody|Iduronate 2 sulfatase 14 kDa chain antibody|Iduronate 2 sulfatase 42 kDa chain antibody|Iduronate 2 sulfatase antibody|Iduronate 2-sulfatase 14 kDa chain antibody|Iduronate sulfatase antibody|Idursulfase antibody|MPS2 antibody|RP23-29M4.1 antibody|SIDS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Iduronate 2 sulfatase antibody, ASA-B0969, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: COLO Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: COLO Cell Lysate
Anti-Iduronate 2 sulfatase antibody, ASA-B0969, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
