More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| isocitrate dehydrogenase 2 (NADP+), mitochondrial | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2 (413-447aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IDH2 |
Protein | Isocitrate dehydrogenase [NADP], mitochondrial |
Uniprot ID | P48735 |
Function | Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion. |
Sequence Similarities | |
Aliases | D2HGA2 antibody|ICD-M antibody|IDH antibody|IDH2 antibody|IDHM antibody|IDHP_HUMAN antibody|IDP antibody|IDPM antibody|Isocitrate dehydrogenase [NADP], mitochondrial antibody|Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody|mNADP-IDH antibody|NADP(+)-specific ICDH antibody|Oxalosuccinate decarboxylase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IDH2 antibody, ASA-B0967, Western blotting
All lanes: Anti IDH2 (ASA-B0967) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
All lanes: Anti IDH2 (ASA-B0967) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
Anti- IDH2 antibody, ASA-B0967, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- IDH2 antibody, ASA-B0967, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue