More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| allograft inflammatory factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Iba1(99-114aa ETFSYPDFLRMMLGKR), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AIF1 |
Protein | Allograft inflammatory factor 1(AIF-1) |
Uniprot ID | P55008 |
Function | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. |
Tissue Specificity | Detected in T-lymphocytes and peripheral blood mononuclear cells. |
Sub-cellular localization | Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. |
Sequence Similarities | |
Aliases | AIF 1 antibody|AIF-1 antibody|Aif1 antibody|AIF1 protein antibody|AIF1_HUMAN antibody|Allograft inflammatory factor 1 antibody|Allograft inflammatory factor 1 splice variant G antibody|allograft inflammatory factor-1 splice variant Hara-1 antibody|balloon angioplasty responsive transcription antibody|BART 1 antibody|G1 antibody|G1 putative splice variant of allograft inflamatory factor 1 antibody|IBA 1 antibody|IBA1 antibody|interferon gamma responsive transcript antibody|Interferon responsive transcript 1 antibody|interferon responsive transcript factor 1 antibody|Ionized calcium binding adapter molecule 1 antibody|Ionized calcium-binding adapter molecule 1 antibody|ionized calcium-binding adapter molecule antibody|IRT 1 antibody|IRT1 antibody|Microglia response factor antibody|MRF1 antibody|Protein g1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Iba1 antibody, ASA-B0960, IHC(P)
IHC(P): Human Tonsil Cancer Tissue
IHC(P): Human Tonsil Cancer Tissue
Anti-Iba1 antibody, ASA-B0960, Western blotting
All lanes: Anti Iba1 (ASA-B0960) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 16KD
All lanes: Anti Iba1 (ASA-B0960) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 16KD
