More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hyaluronoglucosaminidase 3 | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL3(357-371aa SHQRCHGHGRCARRD), different from the related rat sequence by three amino acids, and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HYAL3 |
Protein | Hyaluronidase-3(Hyal-3) |
Uniprot ID | O43820 |
Function | |
Tissue Specificity | Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells. |
Sub-cellular localization | Secreted . Lysosome . |
Sequence Similarities | Belongs to the glycosyl hydrolase 56 family. |
Aliases | Hyal-3 antibody|HYAL3 antibody|HYAL3_HUMAN antibody|Hyaluronidase 3 antibody|Hyaluronidase-3 antibody|Hyaluronoglucosaminidase 3 antibody|Hyaluronoglucosaminidase-3 antibody|LUCA-3 antibody|LUCA14 antibody|LUCA3 antibody|Lung carcinoma protein 3 antibody|Minna14 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HYAL3 antibody, ASA-B0956, Western blotting
Lane 1: 22RV Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: V20S Cell Lysate
Lane 1: 22RV Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: V20S Cell Lysate
Anti-HYAL3 antibody, ASA-B0956, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
