More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| milk fat globule-EGF factor 8 protein | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1(165-180aa NGHEFDFIHDVNKKHK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MFGE8 |
Protein | Lactadherin |
Uniprot ID | Q08431 |
Function | Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. |
Tissue Specificity | Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. |
Sub-cellular localization | Membrane. |
Sequence Similarities | Contains 1 EGF-like domain. |
Aliases | BA 46 antibody|BA46 antibody|Breast epithelial antigen BA46 antibody|Contains Medin antibody|EDIL 1 antibody|EDIL1 antibody|HMFG antibody|hP47 antibody|HsT19888 antibody|Lactadherin antibody|Lactadherin precursor antibody|Medin antibody|MFG E8 antibody|MFG-E8 antibody|MFGE 8 antibody|MFGE8 antibody|MFGE8 protein antibody|MFGM antibody|MFGM_HUMAN antibody|Milk fat globule EGF factor 8 antibody|Milk fat globule EGF factor 8 protein antibody|Milk fat globule-EGF factor 8 antibody|O acetyl disialoganglioside synthase antibody|OAcGD3S antibody|SED1 antibody|SPAG10 antibody|Sperm associated antigen 10 antibody|Sperm surface protein hP47 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Human Milk Fat Globule 1 antibody, ASA-B0947, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human MEGF8, 50.2KD(162aa tag+ S28-A317)
Lane 1: Recombinant Human MEGF8 Protein 10ng
Lane 2: Recombinant Human MEGF8 Protein 5ng
Lane 3: Recombinant Human MEGF8 Protein 2.5ng
Lane 4: Recombinant Human MEGF8 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant human MEGF8, 50.2KD(162aa tag+ S28-A317)
Lane 1: Recombinant Human MEGF8 Protein 10ng
Lane 2: Recombinant Human MEGF8 Protein 5ng
Lane 3: Recombinant Human MEGF8 Protein 2.5ng
Lane 4: Recombinant Human MEGF8 Protein 1.25ng
Anti-Human Milk Fat Globule 1 antibody, ASA-B0947, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
